Can exosomes be refrozen after thawing?

Repeated freeze-thaw cycles damage vesicles and lower particle counts. Thaw once, use what you need, and aliquot the stock on receipt so each tube is thawed a single time.

What temperature should exosomes be stored at?

Follow the storage condition on the certificate and safety data sheet for your batch. As a general rule for research use, long-term storage is cold and frozen, short-term holding is refrigerated, and storage should be consistent.

How can I tell if an exosome batch has degraded?

Typical signs are a fall in particle count, a shift or widening of the size distribution, visible aggregation or cloudiness, and a change in pH. Re-measure against the certificate values if in doubt.

Exosome Storage and Handling: Cold Chain & Freeze-Thaw

Vesicles are lipid bubbles a few hundred times narrower than a cell. They can fuse, aggregate, stick to plastic and leak cargo, and most of that damage happens after the batch leaves the supplier. A few habits protect the quality you paid for.

Receive and record

Check the packaging temperature and the date on receipt, and note both against the certificate of analysis
Record batch number, concentration and expiry in your own log before anything is opened

Aliquot on arrival

The single most useful step. Divide the stock into small, single-use volumes in low-protein-binding tubes so that each portion is thawed once. Label each tube with batch number and date.

Control the temperature

Long term: keep frozen at the temperature given for your batch
Thawing: thaw on ice or in a refrigerator rather than at room temperature or in warm water
Working time: keep tubes cold during use and return unused material to cold storage promptly — or discard it, rather than refreezing

Protect the buffer

Vesicle stability is linked to pH and ionic conditions. Keep your diluent compatible with the one used by the supplier, and avoid strong acids, bases or detergents that dissolve membranes. If you are formulating, run a short compatibility test before committing a large batch.

Choose containers carefully

Particles stick to some plastics and to glass. Low-binding polypropylene tubes reduce losses, especially at low concentration. Avoid repeated pipetting and vigorous vortexing, which shear vesicles.

Know the signs of degradation

Particle count falling against the certificate
Size distribution widening or shifting upward (a sign of aggregation or fusion)
Cloudiness, clumps or visible sediment
pH drifting from the specified range

When in doubt, re-measure size and concentration on a retained aliquot and compare with the certificate.

Key pointAliquot on arrival, thaw once, keep everything cold, use low-binding tubes and watch particle count and size. Those five habits preserve most of a batch’s quality.

For batch-specific storage conditions, see the certificate and safety data sheet supplied with each EXOMIE order, review our quality documentation, or ask the team about stability testing for your formulation.

Storing and handling exosomes.